Single strand confirmation polymorphism (SSCP) detection in exon I of the α-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
نویسندگان
چکیده
The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect α-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon I of the α-lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These α-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism (SNP) markers for association studies and marker assisted selection.
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